Multiple sequence alignment (MSA) is a cornerstone of computational biology, facilitating the exploration of functional, structural and evolutionary relationships among biological sequences.

The identification of evolutionarily constrained sequences is an unbiased approach for finding functional sequences in genomes. However, their identification is strongly affected by upstream analyses.

Roestel, Jessica A., Wiersema, John H., Jansen, Robert K., Borsch, Thomas, and Gruenstaeudl, Michael. 2023. [Dataset] On the importance of correct DNA sequence ...